Thermostable DNA-ligase from Thermofilus aquaticus
Taq DNA Ligase is athermostable NAD+ dependent DNA ligase obtained from E. Coli cells, carrying plasmid with the corresponding gene Thermus aqauticus HB8.
The ferment catalyzes the formation of phosphodiester bond in duplex DNA between 5`- phosphate and 3`- hydroxyl groups, using NAD+ as a co-factor. For the process of DNA chain ligation, oligonucleotides have to be well coupled with the complementary DNA chain and have no gaps.
Taq DNA Ligase works at the temperature 45 - 70 °C.
- Аllele-specific gene typing through Ligase Detection Reaction (LDR) and Ligase Chain Reaction (LCR)
- Mutagenesis with the help of including phosphorylated oligonucleotide during the primer extension amplification.
- Gibson assembly.
- High-fidelity gene synthesis.